Effect of various concentrations of the Percoll® density gradient on the efficiency of selection of viable sperm cell fraction during capacitation of unsexed bull semen
Abstract
The quality and quantity of viable sperm cells influence both fertilization and the overall development of the embryo.
In this regard, the procedure of selection and preparation of sperm for in vitro fertilization, aimed at improving its quantitative and qualitative characteristics, is an integral part of assisted reproductive technologies.
This paper contains the results of research aimed at studying the effect of various concentrations of Percoll in the preparation of a step density gradient on the isolation of the most viable sperm cell fraction during capacitation of bull semen. In the process of using single-, double- and triple-layer density gradient columns in various concentrations, the effectiveness of single-layer 90% Percoll was established for the main indicators of sperm motility.
About the Authors
L. V. Golubets
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
Y. K. Kirikovich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
S. A. Sapsalev
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
Ye. V. Navumenka
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
N. V. Yanuts
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
O. V. Payterova
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
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Review
For citations:
Golubets L.V., Kirikovich Y.K., Sapsalev S.A., Navumenka Ye.V., Yanuts N.V., Payterova O.V. Effect of various concentrations of the Percoll® density gradient on the efficiency of selection of viable sperm cell fraction during capacitation of unsexed bull semen. Zootechnical Science of Belarus. 2025;60(1):21-31. (In Russ.)
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