zootechЗоотехническая наука БеларусиZootechnical Science of Belarus0134-9732Научно-практический центр Национальной академии наук Беларуси по животноводствуzootech-1852Research ArticleГЕНЕТИКА, РАЗВЕДЕНИЕ, СЕЛЕКЦИЯ, БИОЛОГИЯ РАЗМНОЖЕНИЯ И ВОСПРОИЗВОДСТВОВлияние различных концентраций градиента плотности «Перколл» на эффективность отбора жизнеспособной фракции сперматозоидов при капацитации несексированной спермы быковEffect of various concentrations of the Percoll® density gradient on the efficiency of selection of viable sperm cell fraction during capacitation of unsexed bull semenГолубецЛ. В.GolubetsL. V.

Жодино

Zhodino

КириковичЮ. К.KirikovichY. K.

Жодино

Zhodino

СапсалёвС. А.SapsalevS. A.

Жодино

Zhodino

ЯнутьН. В.NavumenkaYe. V.

Жодино

Zhodino

НаменкоЕ. В.YanutsN. V.

Жодино

Zhodino

ПайтероваО. В.PayterovaO. V.

Жодино

Zhodino

Научно-практический центр Национальной академии наук Беларуси по животноводствуScientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding2025100920256012131Copyright © Голубец Л.В., Кирикович Ю.К., Сапсалёв С.А., Януть Н.В., Наменко Е.В., Пайтерова О.В., 20252025Голубец Л.В., Кирикович Ю.К., Сапсалёв С.А., Януть Н.В., Наменко Е.В., Пайтерова О.В.Golubets L.V., Kirikovich Y.K., Sapsalev S.A., Navumenka Y.V., Yanuts N.V., Payterova O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zootech.belal.by/jour/article/view/1852

   Качество и количество живых сперматозоидов оказывают влияние не только на оплодотворение, но и на развитие эмбриона в целом. В этой связи процедура отбора и подготовки спермы к оплодотворению in vitro, направленная на улучшение её количественных и качественных характеристик, является неотъемлемой частью вспомогательных репродуктивных технологий.

   В статье представлены результаты исследований, целью которых явилось изучение влияния различных концентраций Перколла при приготовлении ступенчатого градиента плотности на выделение наиболее жизнеспособной фракции сперматозоидов при капацитации спермы быков.

   В процессе использования одно-, двух- и трёхслойных колонок градиента плотности в различных концентрациях установлена эффективность однослойного 90 % Перколла по основным показателям двигательной активности сперматозоидов.

   The quality and quantity of viable sperm cells influence both fertilization and the overall development of the embryo.

   In this regard, the procedure of selection and preparation of sperm for in vitro fertilization, aimed at improving its quantitative and qualitative characteristics, is an integral part of assisted reproductive technologies.

   This paper contains the results of research aimed at studying the effect of various concentrations of Percoll in the preparation of a step density gradient on the isolation of the most viable sperm cell fraction during capacitation of bull semen. In the process of using single-, double- and triple-layer density gradient columns in various concentrations, the effectiveness of single-layer 90% Percoll was established for the main indicators of sperm motility.

капацитацияПерколлградиент плотностиподвижностьсперматозоидыцентрифугированиеcapacitationPercolldensity gradientmotilitysperm cellscentrifugationReferencesNormal development following in vitro fertilization in the cow / B. G. Bracket, D. Bousquet, M. L. Boice [et. al.] // Biology of Reproduction. – 1982. – Vol. 27(1). – P. 147-158. – DOI: 10.1095/biolreprod27.1.147.Normal development following in vitro fertilization in the cow / B. G. Bracket, D. Bousquet, M. L. Boice [et. al.] // Biology of Reproduction. – 1982. – Vol. 27(1). – P. 147-158. – DOI: 10.1095/biolreprod27.1.147.Pregnancy established in cattle by transfer of embryos derived in vitro from in vitro fertilization of follicular oocytes matured in vitro / K. H. Lu, I. Gordon, M. Gallagher, H. McGovern // Veterinary Record. – 1987. – Vol. 121(11). – P. 159-260. – DOI: 10.1136/vr.121.11.259.Pregnancy established in cattle by transfer of embryos derived in vitro from in vitro fertilization of follicular oocytes matured in vitro / K. H. Lu, I. Gordon, M. Gallagher, H. McGovern // Veterinary Record. – 1987. – Vol. 121(11). – P. 159-260. – DOI: 10.1136/vr.121.11.259.Bovine in vitro fertilization with frozen thawed semen / J. J. Parrish, J. L. Susko-Parrish, M. L. Leibfried-Rutledge [et al.] // Theriogenology. – 1986. – Vol. 25(4). – P. 591-600. – DOI: 10.1016/0093-691x(86)90143-3.Bovine in vitro fertilization with frozen thawed semen / J. J. Parrish, J. L. Susko-Parrish, M. L. Leibfried-Rutledge [et al.] // Theriogenology. – 1986. – Vol. 25(4). – P. 591-600. – DOI: 10.1016/0093-691x(86)90143-3.Barth, A. D. The relationship between sperm abnormalities and fertility / A. D. Barth // Proceedings of the 14<sup>th</sup> Technical Conference on Artificial Insemination and Reproduction National Association of Animal Breeders, Milwaukee, 1992. – P. 47-63.Barth, A. D. The relationship between sperm abnormalities and fertility / A. D. Barth // Proceedings of the 14<sup>th</sup> Technical Conference on Artificial Insemination and Reproduction National Association of Animal Breeders, Milwaukee, 1992. – P. 47-63.Dejarnette, J. M. Accessory sperm: their importance to fertility and embryonic quality, an attempt to alter their numbers in artificially inseminated cattle / J. M. Dejarnette, R. G. Saacke, J. Bame, C. J. Vogler // Journal of Animal Science. – 1992. – Vol. 70(2). – P. 484-491. – DOI: 10.2527/1992.702484x.Dejarnette, J. M. Accessory sperm: their importance to fertility and embryonic quality, an attempt to alter their numbers in artificially inseminated cattle / J. M. Dejarnette, R. G. Saacke, J. Bame, C. J. Vogler // Journal of Animal Science. – 1992. – Vol. 70(2). – P. 484-491. – DOI: 10.2527/1992.702484x.Iodixanol density gradient centrifugation for selecting stallion sperm for cold storage and cryopreservation / G. Stuhtmann, H. Oldenhof, P. Peters [et.al.] // Anim. Reprod. Sci. – 2012. – Vol. 133(3-4). – P. 184–90. – DOI: 10.1016/j.anireprosci.2012.06.017.Iodixanol density gradient centrifugation for selecting stallion sperm for cold storage and cryopreservation / G. Stuhtmann, H. Oldenhof, P. Peters [et.al.] // Anim. Reprod. Sci. – 2012. – Vol. 133(3-4). – P. 184–90. – DOI: 10.1016/j.anireprosci.2012.06.017.Effect of Density-Gradient Sperm Separation Technique on In Vitro Fertilization Potential of Water Buffalo Semen with Low Post-Thaw Motilities / D. Hufana-Duran, P. G. Duran, Y. Kanai [et.al.] // Philippine Agricultural Scientist. – 2005. – Vol. 88(4). – P. 476-483.Effect of Density-Gradient Sperm Separation Technique on In Vitro Fertilization Potential of Water Buffalo Semen with Low Post-Thaw Motilities / D. Hufana-Duran, P. G. Duran, Y. Kanai [et.al.] // Philippine Agricultural Scientist. – 2005. – Vol. 88(4). – P. 476-483.Morrell, J. M. Update on semen technologies for animal breeding / J. M. Morrell // Reprod. Domest. Anim, - 2006. – Vol. 41(1). – P. 63–7. – DOI: 10.1111/j.1439-0531.2006.00621.x.Morrell, J. M. Update on semen technologies for animal breeding / J. M. Morrell // Reprod. Domest. Anim, - 2006. – Vol. 41(1). – P. 63–7. – DOI: 10.1111/j.1439-0531.2006.00621.x.Morrell, J. M. Biometric techniques for improving sperm quality in animal breeding : a review / J. M. Morrell, H. Rodriguez-Martinez // The Open Andrology J. – 2009. – Vol. 1. – P. 1–9.Morrell, J. M. Biometric techniques for improving sperm quality in animal breeding : a review / J. M. Morrell, H. Rodriguez-Martinez // The Open Andrology J. – 2009. – Vol. 1. – P. 1–9.Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates / A. Gloria, A. Carluccio, L. Wegher [et. al.] // Journal of Animal Science and Biotechnology. – 2016. – Vol. 7. – P. 30. – DOI: 10.1186/s40104-016-0088-6Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates / A. Gloria, A. Carluccio, L. Wegher [et. al.] // Journal of Animal Science and Biotechnology. – 2016. – Vol. 7. – P. 30. – DOI: 10.1186/s40104-016-0088-6Single- and two-layer gradient centrifugation in sperm separation: comparison and appraisal / QZ Zhou, CQ Feng, YG Zou [et. al.] // Zhonghua Nan KeXue. – 2010. – Vol. 16(3). – P. 217-219.Single- and two-layer gradient centrifugation in sperm separation: comparison and appraisal / QZ Zhou, CQ Feng, YG Zou [et. al.] // Zhonghua Nan KeXue. – 2010. – Vol. 16(3). – P. 217-219.

The authors declare that there are no conflicts of interest present.