Restoring viability of thawed bovine embryos obtained in-vitro using exocellular substances
Abstract
The viability of thawed bovine embryos obtained in-vitro and reproduced using exocellular substances was studied. It was determined that the use of polyalcohol in the media for cryopreservation, as well as sucrose when filling payette or after thawing at deduction of cryophylactic allows to maintain the viability of 15,8 % of frozen-thawed bovine embryos. A higher tolerance of early bovine embryos to low temperatures is determined using sucrose as compared with trehalose.
About the Authors
L. L. Letkevich
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
A. I. Gandzha
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
V. P. Simonenko
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
I. V. Kirillova
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
E. D. Rakovich
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
O. P. Kurak
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
N. V. Zhurina
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
M. A. Kovalchuk
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
O. V. Burakova
RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal husbandry»
Russian Federation
Russian Federation
References
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Review
For citations:
Letkevich L.L., Gandzha A.I., Simonenko V.P., Kirillova I.V., Rakovich E.D., Kurak O.P., Zhurina N.V., Kovalchuk M.A., Burakova O.V. Restoring viability of thawed bovine embryos obtained in-vitro using exocellular substances. Zootechnical Science of Belarus. 2014;49(1):122-131. (In Russ.)
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