Effect of follicular fluid on the proliferation of somatic cells and bovine embryos obtaining in vitro
Abstract
The effect of follicular fluid on the proliferation of somatic cells and bovine embryos obtaining in vitro was studied. It was determined that the use of 10%-follicular fluid of the total volume as an additive to the medium provides an intensive proliferation of granulosa cells and their potential durable cultivation without signs of cellular aging. To follicular fluid it is recommended to use follicles with diameter of 3-6 mm, the yield of viable embryos at pre-implantation stages increases at 6,6-8,7 % depending on the composition of the culture medium for embryos.
About the Authors
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
L. L. Letkevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. I. Koneva
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Россия
Россия
Y. I. Sheiko
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Россия
Россия
References
1. Treatment of human spermatozoa with follicular fluid can influence lipid content and motility during in vitro capacitation / S. Hamamah [et al] // Fertil. Steril. - 1996. - Vol. 65, № 3. - P. 265-271.
2. Использование фолликулярной жидкости человека на этапе культивирования гамет и эмбрионов в программе ЭКО / В. И. Грищенко [и др.] // Проблемы репродукции. - 1999. - № 6. - C. 43-46.
3. Элдер, К. IVF. Лабораторные процедуры / К. Элдер, С. Эйвери, К. Миллз // Bourn-hallam group. - 1990. - С. 23.
4. Effect of follicular fluid supplementation on the in vitro development of human pre-embryos / R. Hemmings [et al] // Fertil. Steril. - 1994. - Vol. 62, № 5. - P. 1018-1021.
Review
For citations:
Kirillova I.V., Gandzha A.I., Letkevich L.L., Simonenko V.P., Koneva I.I., Sheiko Y.I. Effect of follicular fluid on the proliferation of somatic cells and bovine embryos obtaining in vitro. Zootechnical Science of Belarus. 2013;48(1):104-110. (In Russ.)
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