Condition of Oocyte-cumuli Complexes of Culled Cows and Their In Vitro Fertilization Ability
Abstract
Condition of oocyte-cumuli complexes extracted from ovaries of culled highly productive cows and their correlation with morphofunctional peculiarities of ovaries and ability of in vitro fertilization was studied. It is stated that ovaries at follicular and lutein stage of sex cycle as well as with fresh ovulation contain 60,0-54,4% oocyte-cumuli complexes good for cultivation in vitro that let us get 50,9-24,0% of splitting cells and 15,5% of morul-blastocysts. The same ovaries possess the greatest potentiality for meiosis initiation. Metaphase II is correspondingly reached by 62,2; 50,0 and 60,0% of oocytes. On average 47,1 % of oocytes reach metaphase II stage in 24 hours of in vitro cultivation. The greatest number of degenerated oocytes were observed in ovaries of in-calf cows and gonads with hippofunction - 47,8 and 30 % correspondingly.
About the Authors
L. L. Letkevitch
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. V. Kostikova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
E. D. Rakovitch
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
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Review
For citations:
Letkevitch L.L., Gandzha A.I., Kostikova I.V., Rakovitch E.D. Condition of Oocyte-cumuli Complexes of Culled Cows and Their In Vitro Fertilization Ability. Zootechnical Science of Belarus. 2008;43(1):81-87. (In Russ.)
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