Effect of forskolin as part of cryophylactics on the viability of frozen-thawed cow embryos
Abstract
This paper contains the results of a scientific work aimed at studying the influence of different concentrations of the delipidating agent forskolin as part of protective media on the regenerative capacity of frozen-thawed cattle embryos.
The studies revealed an increase in cryoresistance of frozen-thawed embryos of donor cows when using protective media based on glycerol, ethylene glycol and dimethyl sulfoxide with the introduction of the lipolysis stimulator forskolin at a concentration of 10 μM, which allows for minimal deterioration in the quality of the biomaterial (0.36-0.73 points) and an increase in the yield of embryos suitable for transplantation.
About the Authors
O. V. Paitserava
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
A. I. Budevich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
L. V. Golubets
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
S. N. Paitserav
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
U. K. Kirikovich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
References
1. Effects of forskolin on cryopreservation and embryo development in the domestic cat / T. Rakhmanova, V. Mokrousova, S. Okotrub [et al.] // Theriogenology. – 2023. – Vol. 210(3). – P. 192-198. – DOI: 10.1016/j.theriogenology.2023.07.035.
2. Van Meer, G. Membrane lipids: where they are and how they behave / G. Van Meer, D. Voelker, G. Feigenson // Nat. Rev. Mol. Cell Biol. – 2008 – Vol. 9(2). – P. 112-124. – DOI: 10.1038/nrm2330.
3. Quinn, P. A lipid-phase separation model of low-te mperature damage to biological membranes / P. A. Quinn // Cryobiology. – 1985. – Vol. 22(2). – P. 128–146. – DOI: 10.1016/0011-2240(85)90167-1.
4. The effect of chilling on membrane lipid phase transition in human oocytes and zygotes / Y. Ghetler, Y. Saar, S. Ruth, A. Amir // Human Reproduction. – 2005. – Vol. 20(12). – P. 3385–3389. – DOI: 10.1093/humrep/dei236. Epub 2005 Jul 29.
5. Improved survival of vitrified porcine embryos after partial delipation through chemically stimulated lipolysis and inhibition of apoptosis / H. Men, Y. Agca, L. K. Riley, J. K. Critser // Theriogenology. – 2006. – Vol. 66(8). – P. 2008-2016. – DOI: 10.1016/j.theriogenology.2006.05.018.
6. Birth of a domestic cat kitten produced by vitrification of lipid polarized in vitro matured oocytes / J. Galiguis, M. C. Gómez, S. P. Leibo, C. E. Pope // Cryobiology. – 2014. – Vol. 68(3). – P. 459-466.
7. Seamon, K. B. Forskolin: unique diterque activator of adenylate cyclase in membranes in intact cells / K. B. Seamon, W. Padgett, J. W. Daly // Proc. Natl. Acad. Sci. USA. – 1981. – Vol. 78. – P. 3363–3367. – DOI: 10.1073/pnas.78.6.3363.
8. Honnor, R. C. cAMP-dependent protein kinase and lipolysis in rat adipocytes. II. Definition of steadystate relationship with lipolytic and antilipolytic modulators / R. C. Honnor, G. S. Dhillon, C. Londos // J. Biol. Chem. – 1985. – Vol. 260. – P. 15130–15138.
9. Stralfors P, Belfrage P. Phosphorylation of hormone-sensitive lipase by cyclic AMP-dependent protein kinase. J Biol Chem 1983; 258:15146–15152. – DOI: 10.1016/S0021-9258(17)43785-9
Review
For citations:
Paitserava O.V., Budevich A.I., Golubets L.V., Paitserav S.N., Kirikovich U.K. Effect of forskolin as part of cryophylactics on the viability of frozen-thawed cow embryos. Zootechnical Science of Belarus. 2025;60(1):63-69. (In Russ.)
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