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DNA testing of pigs for PRLR and FSHB genes by PCR-RFLP method

Abstract

   Implementation of effective selection-genetic and biological methods in the selection process contributes to the improvement of native breeds and lines of pigs. The use of DNA markers is a promising method for studying traits that have low heritability and sex-limited manifestations. This paper contains the results of research in which the genetic information of prolactin receptor (PRLR), follicle-stimulating hormone (FSHB) genes was studied in order to establish genotypes preferred for selection, determining the reproductive traits of pigs of various breeds bred at breeding enterprises of the Republic of Belarus, and their use in selection and breeding work. Genotyping of pigs for the studied genes was carried out using the PCR-RFLP method. Polymorphism of PRLR, FSNB genes was observed in all breeding animals belonging to five breeds.

About the Authors

M. A. Kovalchuk
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus

Zhodino



V. P. Simonenko
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus

Zhodino



A. I. Gandzha
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus

Zhodino



N. V. Zhurina
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus

Zhodino



L. L. Letkevich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus

Zhodino



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Review

For citations:


Kovalchuk M.A., Simonenko V.P., Gandzha A.I., Zhurina N.V., Letkevich L.L. DNA testing of pigs for PRLR and FSHB genes by PCR-RFLP method. Zootechnical Science of Belarus. 2025;60(1):31-40. (In Russ.)

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ISSN 0134-9732 (Print)