DEVELOPMENTAL POTENCY OF COW OOCYTES AFTER ICSI PROCEDURE

ICSI (intracytoplasmic sperm injection) is an important step in assisted reproductive technology to overcome the causes of infertility. However, the effectiveness of this technique on farm animals, particularly cows, remains low. In this regard, the study of developmental potency of cow oocytes after the ICSI procedure is relevant. Studies have revealed that cell viability after sperm microinjection procedure is ensured by injection of sperm directly into the oolemma of the oocyte with the position of the first polar body at 6 or 12 o’clock. Aspiration into the microneedle is performed with the head forward, hence transportation to the oolemma after perforation of the membrane is performed with the tail part of the male gamete. The bending angle of the micropipette in its distal part should be 25-30°. A total of 6 dividing cells were obtained from 52 oocytes in the experiment, representing 11.5% of all successful microinjections. Compliance with the specified technical parameters of the procedure of intracytoplasmic sperm injection allows preserving the viability of 19.2% of the operated oocytes with the potency for fertilization and subsequent division. 

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