KINEMATICS OF SPERM MOTILITY OF BILLY GOATS TRANSGENIC FOR RECOMBINANT LACTOFERRIN GENE
Abstract
The article presents the materials of scientific work, the purpose of which was to study the sperm motility of non-transgenic and rLF gene transgenic males of F4-5 generation, taking into account the kinematic parameters of sperm motility. The studies showed that all the billy goats used in the experiment were characterized by biologically complete sperm with fast-moving and linearly progressive sex gametes with a predicted high fertilizing ability. Indicators of sperm motility and kinematics both in transgenic and in common billy goats had the same level of values, which means that the presence of a foreign gene in the organism of transgenic males of F4-5 generation does not affect their reproductive functions.
About the Authors
D. M. Bogdanovich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
E. V. Petrushko
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
Zhodino
References
1. Production of human lactoferrin in animal milk / I. L. Goldman [et al.] // Biochem. Cell. Biol. – 2012. – Vol 90(3). – P. 513–519. DOI: 10.1139/o11-088.
2. Modulation of TDM-induced granuloma pathology by human lactoferrin: A persistent effect in mice / J. K. Actor [et al.] // Biometals. – 2023. – Vol. 36. – P. 603-615. DOI: 10.1007/s10534-022-00434-0
3. Lactoferrin a multiple bioactive protein: An overview / I.A. García-Montoya [et al.] // Biochim Biophys Acta. – 2012. – Vol. 1820. – P. 226-236. DOI: 10.1016/j.bbagen.2011.06.018.
4. Ward, P. P. Multifunctional roles of lactoferrin: A critical overview / P. P. Ward, E. Paz, O. M. Connely // Cell. Mol. Life Sci. – 2005. – Vol. 62. – P. 2540-2548. DOI: 10.1007/s00018-005-5369-8.
5. Получение рекомбинантного лактоферрина человека из молока коз-продуцентов и его физиологические эффекты / В. С. Лукашевич [и др.] // Доклады Нац. акад. наук Беларуси. – 2016. – Т. 60, No 1. – С. 72–81.
6. Goats producing biosimilar human lactoferrin / D. M. Bogdanovich [et al.] // IOP Conf. Ser.: Earth Environ. Sci. – 2021. – Vol. 852. – 12080. DOI: 10.1088/1755-1315/848/1/012080.
7. Влияние интервального доения коз-продуцентов на физико-химические показатели и содержание лактоферрина человека в молоке животных / А. И. Будевич [и др.] // Ученые записки УО "ВГАВМ". – 2023. – Т. 59, вып. 2. – С. 93-98. – DOI 10.52368/2078-0109-2023-59-2-93-98.
8. Isolation of a human lactoferrin biosimilar from frozen milk of goat producers / N. I. Mosolova [et al.] // E3S Web of Conferences : AGRITECH-VIII-2023 : VIII International Conference on Advanced Agritechnologies, Environmental Engineering and Sustainable Development, Krasnoyarsk, Russia, March 29-31, 2023. – Krasnoyarsk, 2023. – Vol. 390. – 02044. DOI 10.1051/e3sconf/202339002044.
9. Expression and performance in transgenic pigs / V. G. Pursel [et al.] // J. Reprod. Fertil. – 1990. – Vol. 40. – P. 235–242.
10. Effects of expression of human or bovine growth hormone genes on sperm production and male reproductive performance in four lines of transgenic mice / A. Bartke [et al.] // J. Reprod. Fertil – 1992. – Vol. 95 (1). – P. 109–118. DOI: 10.1530/jrf.0.0950109.
11. Meliska, C. J. Copulatory behavior and fertility in transgenic male mice expressing human placental growth hormone gene / C. J. Meliska, A. Bartke // J. Androl. – 1997. – Vol. 18(3). – P. 305–311.
12. Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos / M. Bryła [et al.] // Animal Reproduction Science – 2010. – Vol. 118. – P 77–82. DOI: 10.1016/j.anireprosci.2009.06.008.
13. Effects of over-expression of TLR2 in transgenic goats on pathogen clearance and role of 386 up-regulation of lysozyme secretion and infiltration of inflammatory cells / S. Deng [et al.] // BMC Vet. Res. – 2012. – Vol. 196. DOI: 10.1186/1746-6148-8-196.
14. Effects of the 389 TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes / Y. Fang [et al.] // Front. Agr. Sci – 2014. – Vol. 1(4). – P. 314–320. DOI: 10.15302/J-FASE-2014038/
15. Spermatozoa quality of the transgenic rabbit offspring / P. Chrenek [et al.] // Slovak J. Anim. Sci. – 2011. – Vol. 44. – P. 124-128.
16. Evaluating the fitness of human lysozyme transgenic dairy goats: growth and reproductive traits / А. К. Jackson [et al.] // Transgenic Res. – 2010. – Vol. 19(6). – P. 977–986. DOI: 10.1007/s11248-010-9371-z.
17. Growth and reproductive traits of F1-generation transgenic goats for human granulocytecolony stimulating factor / R. Batista [et al.] // Animal Production Science. – 2018. – Vol. 7. – P. 1218. DOI: 10.1071/AN16582.
18. Objective sperm motion analysis to assess dairy bull fertility using computer-aided system- a review / P. Kathiravan [et al.] // Reprod Domest Anim. – 2011. – Vol. 46. – P. 165–172. DOI: 10.1111/j.1439-0531.2010.01603.x.
19. Testing Usability of Butylated Hydroxytoluene in Conservation of Goat Semen / T. A. A. Khalifa [et al.] // Reprod. Domest. Anim. – 2008. – Vol. 43. – P. 525–530. DOI: 10.1111/j.1439-0531.2007.00947.x.
20. Effect of temperature and time after collection on buck sperm quality / H. Kirsten [et al.] // BMC Veterinary Research. – 2019. – Vol. 15. – P. 1-7. DOI: 10.1186/s12917-019-2135-y.
21. Are there differences between methods used for the objective estimation of boar sperm concentration and motility? / F. Sevill [et al.] // Animals. – 2023. – Vol. 13. – P. 1622. DOI: 10.3390/ani13101622
Review
For citations:
Bogdanovich D.M., Petrushko E.V. KINEMATICS OF SPERM MOTILITY OF BILLY GOATS TRANSGENIC FOR RECOMBINANT LACTOFERRIN GENE. Zootechnical Science of Belarus. 2024;59(1):7-13. (In Russ.)
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