EFFECT OF EXPERIMENTAL COMPOSITION OF SYNTHETIC MEDIUM USED FOR CRYOPRESERVATION ON SEMEN QUALITY
Abstract
Various components and freezing modes are used for boar semen diluents, so it is 5 important to determine the effect of their composition and applied cryopreservation doses on semen quality. The paper contains the materials of research, which resulted in the development of a method for assessing the cryoresistance of semen and predicting its fertilizing ability after cryopreservation and defrosting, based on the relationship between the established acceptable limits of changes in the concentration of hydrogen ions, osmotic pressure, motility, integrity of sperm membranes after thawing and fertilizing ability. It has been found that the use of the experimental medium for pre-dilution and freezing of ejaculate allows obtaining values of hydrogen ion concentration of 6.7-6.9 and osmotic pressure of 310-315 mOsm, minimizing the reduction of ejaculate motility after defrosting to 4.5 points, preserving intact up to 51.1% of acrosome membranes and reducing the number of pathological forms of sperm cells to 17-20%. A complex of antibacterial drugs using recombinant human lactoferrin as part of the diluent contributes to high sperm sanitation results.
About the Author
D. M. Bogdanovich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
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Review
For citations:
Bogdanovich D.M. EFFECT OF EXPERIMENTAL COMPOSITION OF SYNTHETIC MEDIUM USED FOR CRYOPRESERVATION ON SEMEN QUALITY. Zootechnical Science of Belarus. 2023;58(1):4-13. (In Russ.)
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