zootechЗоотехническая наука БеларусиZootechnical Science of Belarus0134-9732Научно-практический центр Национальной академии наук Беларуси по животноводствуzootech-1730Research ArticleГЕНЕТИКА, РАЗВЕДЕНИЕ, СЕЛЕКЦИЯ, БИОЛОГИЯ РАЗМНОЖЕНИЯ И ВОСПРОИЗВОДСТВОВЛИЯНИЕ ЭКСПЕРИМЕНТАЛЬНОГО СОСТАВА СИНТЕТИЧЕСКОЙ СРЕДЫ, ПРИМЕНЯЕМОЙ ДЛЯ КРИОКОНСЕРВАЦИИ, НА КАЧЕСТВО СПЕРМЫEFFECT OF EXPERIMENTAL COMPOSITION OF SYNTHETIC MEDIUM USED FOR CRYOPRESERVATION ON SEMEN QUALITYБогдановичД. М.BogdanovichD. M.Научно-практический центр Национальной академии наук Беларуси по животноводствуScientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding202320092023581413Copyright © Богданович Д.М., 20232023Богданович Д.М.Bogdanovich D.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zootech.belal.by/jour/article/view/1730

В составе разбавителей спермы хряков-производителей используются различные компоненты и режимы заморозки, поэтому важно установить влияние их состава и применяемых доз криоконсервации на качество спермы. В статье представлен материал исследований, в результате которых разработан метод оценки криоустойчивости спермы и прогнозирования её оплодотворяющей способности после криоконсервации и дефростации, основанный на взаимосвязи установленных допустимых границ изменения концентрации ионов водорода, осмотического давления, подвижности и целостности мембран спермиев после оттаивания с оплодотворяющей способностью. Установлено, что использование экспериментальной среды для предварительного разбавления и замораживания эякулята позволяет получить величины концентрации ионов водорода 6,7-6,9 и осмотического давления 310-315 мОсм, минимизировать снижение двигательной активности эякулятов после дефростации до 4,5 баллов, сохранить интактными до 51,1 % акросомных мембран и снизить число патологических форм спермиев до17-20 %. Комплекс антибактериальных препаратов с использованием рекомбинантного лактоферрина человека в составе разбавителя способствует высоким результатам санации спермы.

Various components and freezing modes are used for boar semen diluents, so it is 5 important to determine the effect of their composition and applied cryopreservation doses on semen quality. The paper contains the materials of research, which resulted in the development of a method for assessing the cryoresistance of semen and predicting its fertilizing ability after cryopreservation and defrosting, based on the relationship between the established acceptable limits of changes in the concentration of hydrogen ions, osmotic pressure, motility, integrity of sperm membranes after thawing and fertilizing ability. It has been found that the use of the experimental medium for pre-dilution and freezing of ejaculate allows obtaining values of hydrogen ion concentration of 6.7-6.9 and osmotic pressure of 310-315 mOsm, minimizing the reduction of ejaculate motility after defrosting to 4.5 points, preserving intact up to 51.1% of acrosome membranes and reducing the number of pathological forms of sperm cells to 17-20%. A complex of antibacterial drugs using recombinant human lactoferrin as part of the diluent contributes to high sperm sanitation results.

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The authors declare that there are no conflicts of interest present.