EFFECT OF EXOGENOUS FOLLICULOGENESIS REGULATION ON THE EFFICIENCY OF TRANSVAGINAL OOCYTE ASPIRATION
Abstract
The paper presents the results of studies on the effect of folliculogenesis regulation on the efficiency of oocyte aspiration. The analysis of the obtained data shows that the removal of follicles with a diameter over 8 mm does not affect the efficiency of oocyte aspiration. Quantitative and qualitative indicators in the experimental group did not differ from the control one. Removal of the dominant follicle in 72 hours before aspiration, allowed to increase, calculated per one aspiration in comparison with the control, the number of aspirated follicles by 41%, yield of oocytes - by 22.9%, including oocytes suitable for cultivation - by 30.8%. Ovarian stimulation with “FSG Super” preparation increased the number of aspirated follicles by 19.2-39.8%, yield of oocytes - by 32.8-45.9%, including oocytes suitable for maturation - by 39.6-43.7%. “Pluset” preparation increased the number of aspirated follicles by 35.9%, yield of oocytes - by 35.3%, including those suitable for maturation - by 43.9%.
About the Authors
L. V. Golubets
Grodno State Agrarian University
Russian Federation
Russian Federation
A. S. Deshko
Grodno State Agrarian University
Russian Federation
Russian Federation
I. S. Kyssa
Grodno State Agrarian University
Russian Federation
Russian Federation
Y. А. Yakubetc
Grodno State Agrarian University
Russian Federation
Russian Federation
V. I. Belevich
Grodno State Agrarian University
Russian Federation
Russian Federation
А. А. Sekhin
Grodno State Agrarian University
Russian Federation
Russian Federation
V. N. Surmach
Grodno State Agrarian University
Russian Federation
Russian Federation
D. V. Mashtaler
Grodno State Agrarian University
Russian Federation
Russian Federation
References
1. Boni, R. Ovum pick-up in cattle: a 25 yr retrospective analysis / R. Boni // Anim. Reprod. Sci. - 2012. - Vol. 9. - P. 362-369.
2. Некоторые аспекты получения ооцитов крупного рогатого скота путем трансвагинальной пункции фолликулов / В. К. Пестис, Л. В. Голубец, А. С. Дешко, И. С. Кысса, М. В. Попов // Доклады Национальной академии наук Беларуси. - 2016. - Т. 60, № 1. - С. 123-128.
3. Different intervals of ovum pick-up affect the competence of oocytes to support the preimplantation development of cloned bovine embryos / L. J. Ding [et al.] // Mol. Reprod. Dev. - 2008. - Vol. 75. - P. 1710-1715.
4. Bilodeau-Goeseels, S. Cows are not mice: the role of cyclic AMP, phosphodi-esterases, and adenosine monophosphate-activated protein kinase in the maintenance of meiotic arrest in bovine oocytes / S. Bilodeau-Goeseels // Mol. Reprod. Dev. - 2011. - Vol. 78. - P. 734-743.
5. Factors affecting recoverey and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology / F.A. Ward [et al.] // Theriogenology. - 2000. - Vol. 54. - P. 433-446.
6. Cebrian-Serrano, A. Beneficial effect of two culture systems with small groups of embryos on the development and quality of in vitro-produced bovine embryos / A. Cebrian-Serrano, I. Salvador, M. A. Silvestre // Anat Histol Embryol. - 2014. - Vol. 43. - P. 22-30.
7. Holding bovine oocytes in the absence of maturation inhibitors: kinetics of in vitro maturation and effect on blastocyst development after in vitro fertilization / H. Alm [et al.] // Theriogenology. - 2008. - Vol. 70. - P. 1024-1029.
8. Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production / R. De Roover [et al.] // Reprod. Domest. Anim. - 2008. - Vol. 43. - P. 239-245.
9. Nuclear maturation kinetics and in vitro embryo development of cattle oocytes prematured with butyrolactone I combined or not combined with roscovitine / P. Adona [et al.] // Anim. Reprod. Sci. - 2008. - Vol. 104. - P. 389-397.
10. Первый опыт получения эмбрионов крупного рогатого скота in vitro в системе трансвагинальной аспирации ооцитов (ТАО) / В. К. Пестис [и др.] // Весцi Нацыянальнай акадэмii навук Беларусі. Серыя аграрных навук. - 2015. - № 1. - С. 86-91.
Review
For citations:
Golubets L.V., Deshko A.S., Kyssa I.S., Yakubetc Y.А., Belevich V.I., Sekhin А.А., Surmach V.N., Mashtaler D.V. EFFECT OF EXOGENOUS FOLLICULOGENESIS REGULATION ON THE EFFICIENCY OF TRANSVAGINAL OOCYTE ASPIRATION. Zootechnical Science of Belarus. 2020;55(1):99-105. (In Russ.)
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