DNA-TESTING OF PIGS BY MUC4 AND ECR F18/FUT1 GENES AND STUDY OF POLYMORPHISM

DNA-testing was conducted by porcine MUC4 gene and ECR F18/FUT1 gene by PCR-RFLP method. The object of research was pigs of the following breeds: Belarusian Large Whites (BLW), Belarusian Meat (BM), Belarusian black-motley (BBM), Landrace (L), Duroc (D) and Yorkshire (Y), bred in the breeding enterprises of the republic. At the molecular genetic testing gene polymorphism of MUC4 and ECR F18/FUT1 genes was determined with animals of different breeds and sex and age groups (boars-producers, sows, young animals at fattening), represented by alleles MUC4^С, MUC4^G and ECR F18/FUT1^А, ECR F18/FUT1^G, and the following genotypes were identified: MUC4^СС, MUC4^CG, MUC4^GG, ECR F18/FUT1^АА, ECR F18/FUT1^GG and ECR F18/FUT1^AG. Analysis of genetic testing by MUC4 gene showed that Duroc breed is resistant to colibacillosis, all animals had preferred genotype MUC4^CC - without mutations. For other breeds and breed combination the MUC4^CC genotype concentration was: Belarusian black-motley - 53.1 %, Belarusian meat - 56.9 %, BM x L - 63.3 %. The study of genetic structure of breeds in average by ECR F18 / FUT1 gene showed that the lowest frequency of animals with resistant ECR F18/FUT1^АА genotype was observed in populations of the following breeds: Yorkshire - 1.6 %, Belarusian black-motley - 2.0 %, Landrace - 4.5 %, and the highest frequency of genotype ECR F18/FUT1^АА was observed in populations of Duroc breed - 10.3 %, and in animals of BM x L breed combination - 12.5 %.

DNA-testing, pigs, breeds, breeding and hybrid centers, polymorphism, boars, sows, young animals at fattening, genotypes, concentration, frequency, allele, structure, markers, selection

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