Efficiency of the use of prolactin in “in vitro” cattle reproduction system
Abstract
As a result of research an improved nutrient media for the maturation of oocytes and early embryos cultivation with prolactin was developed. It provides improvement of oocytes maturation to the stage of metaphase II by 84.9-88.3 %, while the cleavage rate is 48.8-55.3 %, and the yield of embryos suitable for transplantation - 19.8-25.5 %.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
V. P. Symonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
L. L. Letkevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
O. P. Kurak
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
N. V. Zhurina
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
M. A. Kovalchuk
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
References
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2. Effects of prolactin on intracellular stored calcium in the course of bovine oocyte maturation in vitro / T. I. Kuzmina [et al.] // Theriogenology. - 1999. - Vol. 51. - P. 1363-1374.
3. Prolactin acts as a potent survival factor against C2-ceramideinduced apoptosis in human granulosa cells / C. M. Prolactin [et al.] // Human Reproduction. - 2003. - Vol. 18, N 12. - P. 2672-2677.
4. Cellular localization and changes in expression of prolactin receptor isoform in sheep ovary throughout the estrous cycle / R. A. Picazo [et al.] // Reproduction. - 2004. - Vol. 128. - P. 545-553.
Review
For citations:
Gandzha A.I., Symonenko V.P., Letkevich L.L., Kirillova I.V., Kurak O.P., Zhurina N.V., Kovalchuk M.A. Efficiency of the use of prolactin in “in vitro” cattle reproduction system. Zootechnical Science of Belarus. 2013;48(1):47-52. (In Russ.)
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