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The use of different methods and cryophilic substances for donors’ bovine embryo preparation to direct transplantation

Abstract

The effect of different combinations of supporting media and sucrose concentration on the qualitative composition of thawed embryos of cattle, frozen in 1.4 M glycerol solution or a 1.5 M solution of ethylene glycol was studied, allowing to retain their viability after depreservationin 90,9-100 % of cases.

About the Authors

A. I. Budevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


S. N. Payterov
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


S. A. Sapsalev
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


Y. K. Kirikovich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


T. N. Lukashevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


I. V. Mihedova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


I. I. Budevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


P. E. Sakhonchik
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation


References

1. Leibo, S. P. A one-step method for direct non-surgical transfer of frozen-thawed bovine embryos / S. P. Leibo // Theriogenology. - 1984. - Vol. 21. - P. 767-790.

2. Voelkel, S. A. Direct transfer of frozenthawed bovine embryos / S. A. Voelkel, Y. X. Hu // Theriogenology. - 1992. - Vol. 37(1). - P. 23-37.

3. Leibo, S. P. Direct transfer of cryopreserved cattle embryos in North America / S. P. Leibo, R. J. Mapletoft // In Proc. 17th Annual Convention of the American Embryo Transfer Association (AETA). - 1998. - P. 91-98.

4. Инструкция по искусственному осеменению коров и телок. - Мн., 1999. - 54 с.

5. Методические рекомендации по трансплантации эмбрионов крупного рогатого скота / БелНИИЖ ; сост. : И. И. Будевич [и др.]. - Мн., 2004. - 33 с.


Review

For citations:


Budevich A.I., Payterov S.N., Sapsalev S.A., Kirikovich Y.K., Lukashevich T.N., Mihedova I.V., Budevich I.I., Sakhonchik P.E. The use of different methods and cryophilic substances for donors’ bovine embryo preparation to direct transplantation. Zootechnical Science of Belarus. 2013;48(1):37-46. (In Russ.)

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ISSN 0134-9732 (Print)