Efficiency of the use of somatotropes hormon in in vitro system
Abstract
As a result of research perfected culture media for oocyte maturation and cultivation of early embryos was developed with the use of somatotropes hormon, which provides increased levels of oocyte maturation at the stage of metaphase II to 89,2-91,8 %, while the level of fragmentation is 55,4-57,6 %, and the yield of embryos that are suitable for transplantation - 25,7-27,1 %.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
L. L. Letkevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
References
1. Hull, K. L. Growth hormone: roles in female reproduction / K. L. Hull, S. Harvey // J. Endocrinol. - 2001. - Vol. 168. - P. 1-23.
2. Bartke, A. Role of growth hormone and prolactin in the control of reproduction. What are we learning from transgenic and knock-out animals? / A. Bartke // Steroids. - 1999. - Vol. 64. - P. 598-604.
3. Effect of transitory hyperprolactine-mia on in vitro fertilization of human oocytes / M. C. Mendes [et al.] // J. Reprod. Med. - 2001. - Vol. 46. - P. 444-450.
4. Exogenous growth hormone improves the number of transferable embryos in superovulated ewes / J. Folch [et al.] // Theriogenology. - 2001. - Vol. 55. - P. 1777-1785.
5. Cellular localization and changes in expression of prolactin receptor isoform in sheep ovary throughout the estrous cycle / R. A. Picazo [et al.] // Reproduction. - 2004. - Vol. 128. - P. 545-553.
6. Tarkowski, A. An air-drying method for chromosomal preparation from mouse eggs / A. Tarkowski // Cytogenetic. - 1966. - Vol. 1. - P. 394-400.
Review
For citations:
Gandzha A.I., Letkevich L.L., Simonenko V.P., Kirillova I.V. Efficiency of the use of somatotropes hormon in in vitro system. Zootechnical Science of Belarus. 2012;47(1):27-35. (In Russ.)
Views: 68
Email this article 