Dynamics of quantitative indicators of human lactoferrin in milk of transgenic goats producers
Abstract
It is determined that the average contentof rhLF in milk of transgenic animals amounted to 5,68 g / l as general. Expression of the transgene with product with the target protein in the milk occurred in all animals-producers. The range of average content of recombinant protein for various individuals during the period of lactation ranged from 2,69 to 9,69 g / l., The threshold concentration of lactoferrin in the sample were 18.9 and 0,2 g / l (maximum and minimum concentration , respectively).
About the Authors
A. I. Budevich
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
V. N. Kuznetsova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
N. L. Zaremba
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
D. A. Shemetkov
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
P. E. Sahonchik
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
References
1. Литература
2. Модификация белков молока сельскохозяйственных животных с использованием трансгенеза: биотехнологические возможности, проблемы и перспективы / Л. С. Попов [и др.] // Биотехнология. - 2000. - № 5. - С. 3-18.
3. Циновый, А. В. Физиологические изменения состава молока коров в зависимости от возраста, периода лактации и при первичных нарушениях функции молочной железы : автореф. дисс.. д-ра биол. наук : 03.00.13 / Циновый А.В. - Харьков, 2003. - 26 с.
4. Human milk lactoferrin is a serine protease that cleaves Haemophilus surface proteins at arginine-rich sites / D. R. Hendrixson [et al.] // Mol. Microbiol. - 2003. - Vol. 47. - P. 607-617.
5. Milk composition studies in transgenic goats expressing recombinant human butyrylcholinesterase in the mammary gland / H. Baldassare [et al.] // Transgenic Res. - 2008. - Vol. 17. - P. 863-872.
6. Production and processing of milk from transgenic goat expressing human lysozyme in the mammary gland / E. A. Maga [et al.] // Journal of Dairy Science. - 2006. - Vol. 89. - P. 518-524.
7. Pharmacokinetics of recombinant human leukemia inhibitory factor in sheep / A. Segrave [et al.] // The Journal of pharmacology and experimental therapeutics - 2004. - Vol. 309, № 3. - P. 1085-1092.
8. Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland / E. A. Maga [et al.] // J. Dairy Sci. - 2006. - Vol. 89. - P. 518-524.
9. Production of recombinant albumin by a herd of cloned transgenic cattle / Y. Echelard [et al.] // Transgenic Res. - 2009. - Vol. 8, № 3. - P. 361-376.
Review
For citations:
Budevich A.I., Kuznetsova V.N., Zaremba N.L., Shemetkov D.A., Sahonchik P.E. Dynamics of quantitative indicators of human lactoferrin in milk of transgenic goats producers. Zootechnical Science of Belarus. 2012;47(1):19-26. (In Russ.)
Views: 59
Email this article 