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Safety and Metabolism of Depreserved Oocytes and Early Embryos of Cows

Abstract

Cryopreservation of fresh oocyte-cumuli complexes of cows using 1,5М solution of ethylene glycol ensures cells safety on the level of 100 % and obtaining 9,7 % of embryos on first stages of cleavage. Deep freezing of 2- and 4-cell embryos in 1,4М solution of glycerin with 0,3М of sucrose ensures continuation of cleavage of 9,5 and 8,3 % embryos, correspondingly. Usage of 1,5М of ethylene glycol as a cryoprotector promotes 50 % safety of late blastocysts of a good quality after melting and 25 % after further cultivation.

For citations:


Gandzha A.I., Letkevcih L.L., Simonenko V.P., Lobanok E.S., Nikolskaya V.P. Safety and Metabolism of Depreserved Oocytes and Early Embryos of Cows. Zootechnical Science of Belarus. 2010;45(1):28-35. (In Russ.)

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ISSN 0134-9732 (Print)