Safety and Metabolism of Depreserved Oocytes and Early Embryos of Cows
Abstract
Cryopreservation of fresh oocyte-cumuli complexes of cows using 1,5М solution of ethylene glycol ensures cells safety on the level of 100 % and obtaining 9,7 % of embryos on first stages of cleavage. Deep freezing of 2- and 4-cell embryos in 1,4М solution of glycerin with 0,3М of sucrose ensures continuation of cleavage of 9,5 and 8,3 % embryos, correspondingly. Usage of 1,5М of ethylene glycol as a cryoprotector promotes 50 % safety of late blastocysts of a good quality after melting and 25 % after further cultivation.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
L. L. Letkevcih
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
E. S. Lobanok
SSI "Institute of Biophysics and Cell Engineering of NAS of Belarus"
Russian Federation
Russian Federation
V. P. Nikolskaya
SSI "Institute of Biophysics and Cell Engineering of NAS of Belarus"
Russian Federation
Russian Federation
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Review
For citations:
Gandzha A.I., Letkevcih L.L., Simonenko V.P., Lobanok E.S., Nikolskaya V.P. Safety and Metabolism of Depreserved Oocytes and Early Embryos of Cows. Zootechnical Science of Belarus. 2010;45(1):28-35. (In Russ.)
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