Perfection of Method of Getting Early Embryos at Pre-implant Stages In Vitro
Abstract
As a result of researches it was determined that usage of monolayer of somatic cells of follicle and oviduct as a natural source of hormones and factors of growth let us get 17,3-18,9% of embryos at pre-implant stages. Treatment of embryos by direct polarized light after insemination and during the 7 days of cultivation promoted increase of morul-blastocists outcome at up to 25%. It is determined that АТF content in embryo cells depends on functional condition of embryos and makes 3,0-8,0 pmol/k.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
L. L. Letkevitch
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
E. C. Lobanok
Institute of Biophysics and Cell Engineering National Academy of Sciences of Belarus
Россия
Россия
V. P. Nikol’Skaya
Institute of Biophysics and Cell Engineering National Academy of Sciences of Belarus
Россия
Россия
References
1. Генетика, селекция и биотехнология в скотоводстве / под ред. М. В. Зубец, В. П. Буркат. - Киев : БМТ, 2001. - 723 с.
2. Wijayagunawardane, M. P. Prostaglandin E2, prostaglandin F2 alpha and endothelin-1 production by cow oviductal epithelial cell monolayers: effect of progesterone, estradiol 17 beta, oxytocin and luteinizing hormone / M. P. Wijayagunawardane, A. Miyamoto, K. Sato // Theriogenology. - 1999. - Vol. 52. - P. 791-801.
3. Сингина, Г. Н. Исследования развития эмбрионов крупного рогатого скота in vitro / Г. Н. Сингина, О. Л. Шатайло // Современные достижения и проблемы биотехнологии с.-х. животных : материалы 6-й Междунар. конф. (19-20 дек. 2006 г.). - Дубровицы, 2006. - С. 167-171.
4. Swain, J. E. [et al.] // Reproduction. - 2002. - Vol. 123. - P. 253-260.
Review
For citations:
Gandzha A.I., Letkevitch L.L., Simonenko V.P., Kirillova I.V., Lobanok E.C., Nikol’Skaya V.P. Perfection of Method of Getting Early Embryos at Pre-implant Stages In Vitro. Zootechnical Science of Belarus. 2009;44(1):28-37. (In Russ.)
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