Perfection of Method of Getting Early Embryos at Pre-implant Stages In Vitro
Abstract
As a result of researches it was determined that usage of monolayer of somatic cells of follicle and oviduct as a natural source of hormones and factors of growth let us get 17,3-18,9% of embryos at pre-implant stages. Treatment of embryos by direct polarized light after insemination and during the 7 days of cultivation promoted increase of morul-blastocists outcome at up to 25%. It is determined that АТF content in embryo cells depends on functional condition of embryos and makes 3,0-8,0 pmol/k.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
L. L. Letkevitch
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Russian Federation
Russian Federation
E. C. Lobanok
Institute of Biophysics and Cell Engineering National Academy of Sciences of Belarus
Russian Federation
Russian Federation
V. P. Nikol’Skaya
Institute of Biophysics and Cell Engineering National Academy of Sciences of Belarus
Russian Federation
Russian Federation
References
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Review
For citations:
Gandzha A.I., Letkevitch L.L., Simonenko V.P., Kirillova I.V., Lobanok E.C., Nikol’Skaya V.P. Perfection of Method of Getting Early Embryos at Pre-implant Stages In Vitro. Zootechnical Science of Belarus. 2009;44(1):28-37. (In Russ.)
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