In vitro Production of Embryos Using Monolayer of Granulosis Cells
Abstract
Conditions of usage of monolayer granulosis cells in technology of cattle embryos in vitro production were developed. It let us get 18,4-19,2% of embryos at pre-implant stages. Addition of follicular liquid to the environment of granulosis cells cultivation with pre-sowing treatment of cells by concentrate of antibiotics let us get long-term (up to 40 days) culture of granulosis cells.
About the Authors
A. I. Gandzha
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
L. L. Letkevitch
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
V. P. Simonenko
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. V. Kirillova
RUE "Scientific and Practical Center of the National Academy of Sciences of Belarus on Animal Husbandry"
Россия
Россия
I. I. Koneva
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Россия
Россия
O. V. Kvitko
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Россия
Россия
Ya. I. Sheyko
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Россия
Россия
References
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2. Apoptosis in cumulus cells, but not in oocytes, may influence bovine embryonic developmental competence / Y. Q. Yuan [et al.] // Theriogenology. - 2005. - Vol. 63(8). - P. 2147-2163.
3. Abilities of cumulus and granulosa cells to enhance the developmental competence of bovine oocytes during in vitro maturation period are promoted by midkine; a possible implication of its apoptosis suppressing effects / S. Ikeda [et al.] // Reproduction. - 2006 - Vol. 132. - Р. 549-557.
4. Gutierrez, C. G. Development of a long-term bovine granulosa cell culture system: induction and maintenance of estradiol production, response to follicle- stimulating hormone, and morphological characteristics / C. G. Gutierrez, B. K. Campbell, R. Webb // Biology of Reproduction. - 1997. - Vol. 56. - Р. 608-616
Review
For citations:
Gandzha A.I., Letkevitch L.L., Simonenko V.P., Kirillova I.V., Koneva I.I., Kvitko O.V., Sheyko Ya.I. In vitro Production of Embryos Using Monolayer of Granulosis Cells. Zootechnical Science of Belarus. 2009;44(1):20-28. (In Russ.)
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