CONDITIONS FOR PREPARATION OF BOVINE SPERMATOZOA FOR INTRACYTOPLASMIC INJECTION
Abstract
This paper presents the data of the work, the purpose of which was to study the conditions for preparation of bovine spermatozoa for intracytoplasmic injection. As a result of the research, the conditions for successful preparation of frozen-thawed semen of Holsteinized bulls for intracytoplasmic injection were determined. It has been established that the use of a culture medium for capacitation based on Tyrode’s solution containing cresacin at a concentration of 3 mg/ml or recombinant human lactoferrin at a concentration of 1 mg/ml ensures the absence of proximal drops in 100% of spermatozoa after capacitation, distal drops in 94.6-95.2% of spermatozoa, curved tails in 99.6-100% of spermatozoa, as well as obtaining 85.5-86.5% of morphologically normal spermatozoa without defects in the head, body and tail, and overall gamete motility indices at the level of 68-77%, while maintaining the optimal parameters of speed, trajectory and amplitude.
About the Authors
L. L. Letkevich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
V. P. Simonenko
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
A. I. Gandzha
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
I. V. Kirillova
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
E. D. Rakovich
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
N. V. Zhurina
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
M. A. Kovalchuk
Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Belarus
Belarus
References
1. Цитологические, цитогенетические показатели гамет, физиологическое состояние яичников коров и жизнеспособность полученных эмбрионов / В. П. Симоненко [и др.] // Актуальные проблемы интенсивного развития животноводства: сб. науч. тр. – Горки : БГСХА, 2020. – Вып. 23, ч. 1. – С. 13-21.
2. Application of intracytoplasmic sperm injection to the embryo production in aged cows / F. Magata [et al.] // J. Vet. Med. Sci. – 2019. – Vol. 81(1). – P. 84–90.
3. Kolbe, T. Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI) / T. Kolbe, W. Holtz // Animal Reproduction Science. – 2000. – Vol. 64. – P. 97–101.
4. Мужское бесплодие и нарушение структурной организации хроматина сперматозоидов / О. А. Воробьёва [и др.] // Проблемы репродукции. – 2005. – Т. 6. – С. 56–62.
5. Баранов, В. С. Цитогенетика эмбрионального развития человека / В. С. Баранов. – СПб., 2007. – 640 с.
6. Фертильность сперматозоидов и состояние хроматина: методы контроля (обзор) / В. А. Багиров [и др.] // Сельскохозяйственная биология. – 2012. – № 2. – С. 3-13.
7. Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection? / M. Franzek [et al.] // 98 Journal of Assisted Reproduction and Genetics. – 2015. – Vol. 32, № 5. – P. 771-779. DOI: 10.1007/s10815-015-0462-x
8. Ball, P. J. H. Reproduction in cattle / P. J. H. Ball, A. R. Peters. – 3th ed. – Oxford : Blackwell Publishing Ltd, 2004. – 250 p.
9. Клещев, М. А. Оценка морфологических аномалий сперматозоидов у быков – производителей / М. А. Клещев, В. Л. Петухов, Л. В. Осадчук // Эффективное животноводство. – 2018. № 3. – С. 69-71.
10. Биологические показатели спермы быков-производителей in vitro / В. П. Симоненко [и др.] // Актуальные проблемы интенсивного развития животноводства : сб. науч. тр. – Горки : БГСХА, 2021. – Вып. 24, ч. 1. – С. 70-77.
Review
For citations:
Letkevich L.L., Simonenko V.P., Gandzha A.I., Kirillova I.V., Rakovich E.D., Zhurina N.V., Kovalchuk M.A. CONDITIONS FOR PREPARATION OF BOVINE SPERMATOZOA FOR INTRACYTOPLASMIC INJECTION. Zootechnical Science of Belarus. 2023;58(1):102-110. (In Russ.)
Views:
79
Email this article 