MORPHOLOGICAL FEATURES OF OOCYTE-CUMULUS COM- PLEXES OBTAINED BY TRANSVAGINAL OOCYTE PICK UP
Abstract
The use of transvaginal aspiration of oocytes (TAO) with their subsequent fertilization and thus obtaining embryos in vitro is largely dictated by the need of the livestock breeding industry for continuous genetic improvement of dairy and beef cattle. This area has become even more important after the introduction of genomic selection. In the technology of life-time in vitro production of bovine embryos, the qualitative and quantitative indicators of the initial population of viable oocytes determine the successful yield of blastocysts suitable for subsequent embryotransfer or cryopreservation. This paper describes the results of studies on the morphological features of oocyte-cumulus complexes obtained by transvaginal oocyte pick up (OPU). Analysis of the data obtained shows that the number of unsuitable oocytes during retrieval was approximately at the same level – 5.8% and 8.2% in the control and experimental groups, respectively. The proportion of oocytes with a layer of cumulus cells from one to three increased compared to the control by 27.1 and 17.7 p.p., respectively, and with a multilayer cumulus decreased by 47.2 p.p. The level of oocytes with a multilayer dense cumulus decreased by 10.2 p.p., and the level with a multilayer loose cumulus increased by 10.2 p.p.
About the Authors
L. V. Golubets
Scientific and Practical Center of the National Academy of Sciences BelSimex JLLC, Samokhvalovichi agro-townof Belarus for Animal Breeding
Russian Federation
Russian Federation
A. S. Deshko
Grodno State Agrarian University
Belarus
Belarus
I. S. Kyssa
BelSimex JLLC
Belarus
Belarus
Samokhvalovichi agro-town
T. Y. Dragun
Grodno State Agrarian University
Belarus
Belarus
M. A. Sekhina
Grodno State Agrarian University
Belarus
Belarus
D. N. Kharitonik
Grodno State Agrarian University
Belarus
Belarus
References
1. Кысса, И. С. Ускоренное воспроизводство высокопродуктивных племенных животных в молочном и мясном скотоводстве на основе новых биотехнологических методов : автореф. дисс. … д-ра биол. наук / Кысса И.С. – Дубровицы, 2000. – 42 с.
2. . Эффективность получения ооцитов методом трансвагинальной аспирации у коровдоноров / В. К. Пестис [и др.] // Сельское хозяйство – проблемы и перспективы : сб. науч. тр. – Гродно : ГГАУ, 2014. – Т. 26 : Зоотехния. – С. 218-225.
3. Карымсаков, Т. Н. Сравнительные результаты приживляемости сексированных эмбрионов, полученные методами in vivo и in vitro / Т. Н. Карымсаков, Д. М. Бекенов, А. А. Спанов // Молочное и мясное скотоводство. – 2017. – № 6. – С. 9–11.
4. Ultrasound-guided follicle aspiration: the collection of bovine cumulus-oocyte complexes from ovaries of slaughtered or live cows / S. R. Ashimoto [et al.] // Theriogenology. – 1919. – Vol. 51. – P. 757-765. 5. Вilodeau-Goeseels, S. Effects of oocyte quality on development and transcriptional activity in early bovine embryos / S. Вilodeau-Goeseels, P. Panich // Anim. Reprod. Sci. – 2002. – Vol. 71. – P. 143-155.
5. Blondin, P. Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes / P. Blondin, M. A. Sirard // Mol. Reprod. Dev. – 1995. – Vol. 41. – P. 54-62.
6. Bols, P. Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes / P. Bols [et al.] // Theriogenology. – 1996. – Vol. 45. – P. 1001-1014.
7. Callesen, H. Ultrasonically guided aspiration of bovine follicular oocytes / H. Callesen, T. Greve, F. Christensen // Theriogenology. – 1987. – Vol. 27. – P. 217.
8. Transvaginal ultrasound guided follicular aspiration of bovine oocytes / M. C. Pieters [et al.] // Theriogenology. – 1991. – Vol. 35. – P. 857–862. 9. Embryo production by ovum pick up from live donors / C. Galli [et al.] // Theriogenology. – 2001. – Vol. 55. – P. 1341-1357.
9. In vitro developmental competence of embryos derived from transvaginal ultrasound oocyte aspiration in cycling cows and from slaughterhouse ovaries / I. Gerz [et al.] // ESDAR Newsletter 6:Proceedings of the 5th Annual Conference of the European Society for Domestic AnimalReproduction. 13-15. September, 2001, Vienna, p. 27.
10. Gordon, I. Laboratory production of cattle embryos : monography / I. Gordon. – Dublin, 2003. – 548 p.
11. Factors affecting oocyte quality and quantity in commercial application of embryotechnologies in the cattle breeding industry / J. S. Merton [et al.] // Theriogenology. – 2003. – Vol. 59. – P. 651-674.
12. Aspiration of bovine oocytes during transvaginal ultrasound scanning of ovaries / M. C. Pieterse [et al.] // Theriogenology. – 1988. – Vol. 30. – P. 751-762.
Review
For citations:
Golubets L.V., Deshko A.S., Kyssa I.S., Dragun T.Y., Sekhina M.A., Kharitonik D.N. MORPHOLOGICAL FEATURES OF OOCYTE-CUMULUS COM- PLEXES OBTAINED BY TRANSVAGINAL OOCYTE PICK UP. Zootechnical Science of Belarus. 2023;58(1):49-55. (In Russ.)
Views: 79
Email this article 