EFFICIENCY OF VARIOUS CRYOPHYLACTIC COMPLEXES FOR FREEZING MAMMALIAN EMBRYOS OBTAINED IN VITRO
Abstract
The paper presents the results of study of effects of various complexes of cryophylactics on safety and viability of mammalian embryos obtained in vitro during freezing and thawing. It was determined that mixture of 20% DMSO solution and 20% EG solution along with 0.7 M sucrose used as cryoprotector allows to ensure the maximum embryos safety and maximum level of cleavage after thawing - 88.2 and 80.0%, respectively. Complex of cryophylactics consisting of 20% solution of DMSO, 20% EG solution and 30% solution of sucrose shows the highest protective properties: using this complex allows for preservation of embryos after thawing at the maximum highest level among all the studied complexes cryophylactics (88.9%) with a high level of embryo material cleavage after depreservation (75.0%).
Keywords
крупный рогатый скот,
лабораторные мыши,
ооцит,
эмбрион,
яичник,
cattle,
laboratory mice,
oocyte,
embryo,
ovary
About the Authors
D. M. Bogdanovich
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
S. N. Paitserau
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
S. А. Sapsalev
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
Y. K. Kirikovich
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
V. V. Zhdanovich
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
О. V. Paitserava
Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding
Russian Federation
Russian Federation
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Review
For citations:
Bogdanovich D.M., Paitserau S.N., Sapsalev S.А., Kirikovich Y.K., Zhdanovich V.V., Paitserava О.V. EFFICIENCY OF VARIOUS CRYOPHYLACTIC COMPLEXES FOR FREEZING MAMMALIAN EMBRYOS OBTAINED IN VITRO. Zootechnical Science of Belarus. 2020;55(1):33-41. (In Russ.)
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