DETERMINING AVAILABLE LYSINE IN PROTEIN FEEDS FOR PIGS

High digestibility ratios of crude protein (at the level of 80.8-83.4) and nitrogen-free extractives (86.4-91.1) of all high-protein feeds were determined. However, there were differences between the cultures themselves: thus, availability of crude protein in rape seeds was 1.6%, and crude fat 6.6% lower than the availability of these nutrients in soybeans. Digestibility of protein from feed beans made 80.8%, of fat - 68.8%, and was the lowest of all the cultures researched. According to the amount of the first limiting amino acid (lysine), local protein feeds showed 1.3-2.0 times higher indices of its content requirement in the “ideal protein”. All the samples, excluding rapeseed, were deficient in methionine and cystine. Deficiency of 25% of threonine was determined in forage beans, and in lupine grain very little tryptophan level was determined. According to the level of valine, leucine, isoleucine, arginine, histidine, phenylalanine and tyrosine, all the protein cultures had a positive balance in comparison with the “ideal protein”. Assessment of availability of lysine in protein feeds in vitro using 2,4-dinitrofluorobenzene showed that the in vitro digestibility of lysine in all the samples was 1.1-10.5% higher than the table values of standardized ileal digestibility of feeds. Lysine loss in rapeseed seeds amounted to 0.437 mg per 100 g of feed, in narrow-leaved lupine - 0.197, field pea (maple pea) - 0.247 and forage beans - 0.132 mg. Discrepancies between the results obtained and table data are explained by the fact that the digestibility of protein and amino acids in vivo depends on the loss of endogenous protein, and these losses are not considered in the in vitro method.

in vitro, in vivo, field pea, availability of amino acids, in vitro, in vivo, fodder beans, lysine, rapeseed

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