CRYOPRESERVATION AND CRYOTOLERANCE OF OOCYTES OF FARM ANIMALS
Abstract
As a result of joint experiments, new knowledge has been obtained on mechanisms of cryotolerance of cow oocytes, on the basis of which the method of obtaining embryos from intra-ovary vitified gametes has been modernized by using preventive VSV-diagnostics and introducing 0.001 % of highly disperse silica into the oocyte ripening medium. The preservation of thawed oocytes made 58.1-84.4 %, maturation - 44.4-60.0 % with the yield of splitting embryos of 7.1-18.2 %, including 5.5 % of blastocysts.
Keywords
About the Authors
А. I. Gandzha
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
L. L. Letkevich
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
Т. I. Kuzmina
FSBRI «All-Russian research institute of genetics and breeding farm animal»
Russian Federation
Russian Federation
V. P. Simonenko
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
I. V. Kirillova
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
Е. D. Rakovich
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
О. P. Kurak
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
N. V. Zhurina
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
М. А. Kovalchuk
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
L. V. Gluschenko
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
О. V. Burakova
RUE «Scientific and practical center of the National academy of sciences of Belarus for Animal husbandry»
Russian Federation
Russian Federation
References
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Review
For citations:
Gandzha А.I., Letkevich L.L., Kuzmina Т.I., Simonenko V.P., Kirillova I.V., Rakovich Е.D., Kurak О.P., Zhurina N.V., Kovalchuk М.А., Gluschenko L.V., Burakova О.V. CRYOPRESERVATION AND CRYOTOLERANCE OF OOCYTES OF FARM ANIMALS. Zootechnical Science of Belarus. 2017;52(1):46-52. (In Russ.)
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