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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">zootech</journal-id><journal-title-group><journal-title xml:lang="ru">Зоотехническая наука Беларуси</journal-title><trans-title-group xml:lang="en"><trans-title>Zootechnical Science of Belarus</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0134-9732</issn><publisher><publisher-name>Научно-практический центр Национальной академии наук Беларуси по животноводству</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.47612/0134-9732-2022-57-1-60-68</article-id><article-id custom-type="elpub" pub-id-type="custom">zootech-1671</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИКА, РАЗВЕДЕНИЕ, СЕЛЕКЦИЯ, БИОТЕХНОЛОГИЯ РАЗМНОЖЕНИЯИ ВОСПРОИЗВОДСТВО</subject></subj-group></article-categories><title-group><article-title>УСЛОВИЯ ПОДГОТОВКИ ООЦИТОВ КРУПНОГО РОГАТОГО СКОТА К ПРОВЕДЕНИЮ МИКРОМАНИПУЛЯЦИЙВНЕ ОРГАНИЗМА</article-title><trans-title-group xml:lang="en"><trans-title>CONDITIONS FOR PREPARATION OF CATTLE OOCYTES FOR IN VITRO MICROMANIPULATION</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ганджа</surname><given-names>А. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Gandzha</surname><given-names>A. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Леткевич</surname><given-names>Л. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Letkevich</surname><given-names>L. L.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Симоненко</surname><given-names>В. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Simonenko</surname><given-names>V. P.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кириллова</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kirillova</surname><given-names>I. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ракович</surname><given-names>Е. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Rakovich</surname><given-names>Е. D.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Журина</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhurina</surname><given-names>N. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковальчук</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalchuk</surname><given-names>M. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Научно-практический центр Национальной академии наук Беларуси по животноводству</institution></aff><aff xml:lang="en"><institution>Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>07</day><month>09</month><year>2022</year></pub-date><volume>57</volume><issue>1</issue><fpage>60</fpage><lpage>68</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ганджа А.И., Леткевич Л.Л., Симоненко В.П., Кириллова И.В., Ракович Е.Д., Журина Н.В., Ковальчук М.А., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Ганджа А.И., Леткевич Л.Л., Симоненко В.П., Кириллова И.В., Ракович Е.Д., Журина Н.В., Ковальчук М.А.</copyright-holder><copyright-holder xml:lang="en">Gandzha A.I., Letkevich L.L., Simonenko V.P., Kirillova I.V., Rakovich Е.D., Zhurina N.V., Kovalchuk M.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://zootech.belal.by/jour/article/view/1671">https://zootech.belal.by/jour/article/view/1671</self-uri><abstract><p>При оплодотворении ооцитов крупного рогатого скота посредством интрацитоплазматической инъекции сперматозоида сперматозоид вводится непосредственно в цитоплазму яйцеклетки, что позволяет преодолеть вне организма три естественных барьера. При угрозе утраты высокоценного генетического материала мужской или женской особи это может явиться единственным способом сохранения ценных наследственных качеств с высокой окупаемостью затрат, а также послужит фундаментом для дальнейшего развития генной инженерии. В связи с этим в лаборатории молекулярной биотехнологии и ДНК-тестирования РУП «Научно-практический центр Национальной академии наук Беларуси по животноводству», в которых разработаны и изучены условия подготовки ооцитов крупного рогатого скота к проведению микроманипуляций вне организма. Установлено, что предпочтительно использовать яичники в лютеиновой и фолликулярной фазах.</p></abstract><trans-abstract xml:lang="en"><p>When cattle oocytes are fertilized through intracytoplasmic sperm injection, the sperm is injected directly into the cytoplasm of the egg cell, allowing three natural barriers to be overcome outside the body. If the loss of highly valuable genetic material in a male or female parent is threatened, this may be the only way to preserve valuable hereditary traits with a high cost recovery, and it may also serve as the basis for further development of genetic engineering. In this regard, the Laboratory for Molecular Biotechnology and DNA Testing of the RUE “Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding” has developed and studied conditions for preparation of cattle oocytes for in vitro micromanipulation. It has been established that it is preferable to use the ovaries in the luteal and follicular phases.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>ооцит</kwd><kwd>капацитация</kwd><kwd>подвижность</kwd><kwd>скорость</kwd><kwd>траектория</kwd><kwd>амплитуда</kwd><kwd>экстракорпоральное оплодотворение</kwd><kwd>капацитация</kwd><kwd>спермии</kwd></kwd-group><kwd-group xml:lang="en"><kwd>oocyte</kwd><kwd>capacitation</kwd><kwd>motility</kwd><kwd>speed</kwd><kwd>trajectory</kwd><kwd>amplitude</kwd><kwd>in vitro fertilization</kwd><kwd>sperm cells</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Цитологические, цитогенетические показатели гамет, физиологическое состояние яичников коров и жизнеспособность полученных эмбрионов / В. П. 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